|
|
|
| Registros recuperados: 9.178 | |
|
|
| MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
| Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
| Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
| |
|
|
| Kuhn,Jennifer; Müller,Hagen; Salzig,Denise; Czermak,Peter. |
| Background The purpose of this work was to find a rapid method for glycerol detection during microbial fermentations. The method requirements were, first, to avoid sample pretreatment, and second, to measure glycerol precisely especially out of fermentation broth. Results This was achieved by combining two reaction principles - the Malaprade reaction and the Hantzsch reaction. In the Malaprade reaction, glycerol is converted into formaldehyde. This forms a dye in the Hantzsch reaction after which adsorption is than detected. The subsequent assay was investigated with two different fermentation media, a chemically undefined and a chemically defined media, used for Pichia pastoris fermentation. In both media, as well as in real fermentation samples, glycerol... |
| Tipo: Journal article |
Palavras-chave: Glycerol determination; Fermentation broth; Hantzsch reaction; Malaprade reaction. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300017 |
| |
|
| |
|
| |
|
| |
|
|
| Jacomo,A. L; Martinez,C. A. R; Saleh,S. O; Andrade,M; Akamatsu,F. E.. |
| Unlike the venous system, variations in arterial anatomy are less frequent and most of them affect visceral arteries. In limbs, variations of the brachial artery are the most reported and at least six different patterns have been described so far. The commonest is the superficial brachial artery which lies superficially to the median nerve. Much less prevalent are the high origin of the radial artery (brachioradial artery) or the existence of a doubled brachial artery (accessory brachial artery). We present a previously undescribed pattern of brachial artery variation. During dissection of the right upper limb of a 60 year-old male embalmed cadaver, we found the bifurcation of the brachial artery in the proximal portion of the middle third of the arm. Its... |
| Tipo: Journal article |
Palavras-chave: Artery; Brachial; Variation; Anatomy. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200027 |
| |
|
| |
|
| |
|
|
| Zheng,Yong; Yuan,Qianqian; He,Xin; Lei,Yueshan. |
| Any compression of testicular arteries may lead to loss of spermatogenesis and gonadal hormone production, existence of the variational arteries is accountable in cases of vasoligation, orchidopexy and other surgical approach on them. Anomalies of the testicular blood arteries were observed during dissection of the pelvic cavity in a 68-year-old male cadaver. This report describes a very rare case of lack of testicular arteries. For the blood supply to the testis, thick deferential arteries form some vascular winding and loops and course accompanied by deferent duct to the testis. This case report would serve as ray of light for knowledge of the possible variations of the testicular arteries during surgical procedures. |
| Tipo: Journal article |
Palavras-chave: Deferential artery; Variation; Umbilical artery. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300022 |
| |
|
| |
|
| |
|
|
| Liu,Chongbin; Xia,Chengqiang; Xie,Zhaohui; Jiao,Yang; She,Qiusheng. |
| The present paper deals with a histological study of the blood cells of Bufo Bufo gargarizans in different months: January, March, May, July and October. The methods used are by routine blood smear in Wright stain and observation in vivo. We found that in smears and in vivo two main types of cells of the red cells: mitotic as well as amitotic. While amitotic occurs all the year round, particularly in July, mitosis so far had been seen only in July. It is also found that there are plenty of neutrophils in the blood cells of Bufo Bufo gargarizans, furthermore, the nuclei of these cells are polymorphic, especially in January and March. Meanwhile, the concentration of red cells was lowest in May and highest in January; The concentration of white blood cells... |
| Tipo: Journal article |
Palavras-chave: Bufo Bufo gargarizans; Blood cells; Annual changes. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400022 |
| |
|
|
| Kenneth W,Bruland. |
| This presentation is a review of the chemistries of redox sensitive elements within suboxic regions of extreme oxygen minimum zones. Suboxic regions are defined as extreme oxygen minimum zones with dissolved oxygen < 10 µmol kg-1 (and likely <5 µmol kg-1), and with clear evidence of nitrate reduction, but not sulfate reduction. These are regions with nitrate deficits > 10 µmol kg-1 or N* values of _10 µmol kg-1. Suboxic regions predictably occur in the tropical and subtropical eastern Pacific and the Arabian Sea. These suboxic zones are important in the global biogeochemistry of nitrogen and act as an important sink for fixed nitrogen. Minor and trace element redox couples such as iodate/iodide and leachable particulate Mn/dissolved Mn undergo... |
| Tipo: Journal article |
Palavras-chave: Nitrate; Iodine; Manganese; Iron. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382006000300003 |
| |
|
| |
|
| |
|
|
| Niggol Seo,S; Mendelsohn,Robert. |
| This study estimates the impact of climate change on South American agriculture taking into account farm adaptations. The study used a Ricardian analysis of 2300 farms to explore the effects of global warming on land values. In order to predict climate change impacts for this century, were examined climate change scenarios predicted by three Atmospheric Oceanic General Circulation Models (AOGCM): the Canadian Climate Center (CCC), the Centre for Climate System Research (CCSR), and the Parallel Climate Model (PCM) models. Several econometric specifications were tested, and five separate regressions were run: for all farms, small household farms, large commercial farms, rainfed farms, and irrigated farms. Farmland values will decrease as temperature... |
| Tipo: Journal article |
Palavras-chave: Climate change; Agriculture; Ricardian approach; South America. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100007 |
| |
|
| |
|
| |
|
|
| Mustafa Harlioglu,Muzaffer. |
| This study compares the morphology of rostrum, pereipods 1,2,4 and mouthparts of juvenile Astacus leptodactylus with those oí Pacifastacus leniusculus. Differences in morphology were observed, in particular with regard to the mouthparts e.g. including setal armature and number of teeth on the mandible. In general, the shape of the rostra in the two species is similar in that both taper to a point with a pair of sharp spines distally. Laterally the rostrum of A. leptodactylus is bordered by a regular row of setae, which is not so well defined in P leniusculus. The observations also showed that in addition to an increase in size, changes in morphology in the feeding apparatus between the developmental stages of the two species were present. It was concluded... |
| Tipo: Journal article |
Palavras-chave: Appendages; Astacus; Crayfish; Mouthparts; Pacifastacus; Scanning electrón microscope. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400040 |
| |
|
|
| Andrés,Silvia; García,José M; Compés,Raúl. |
| S. Andrés, J.M. García, and R. Compés. 2013. A scenario for the conversion of Common Agricultural Policy payments in Spain after the 2011 legislative proposals. Cien. Inv. Agr. 40(2):291-306. This paper evaluates the impact of a new model of agricultural policy outlined in legislative proposals issued by the European Commission in October 2011 and estimates its effects on agricultural aid in Spanish Autonomous Communities compared with 2009. Therefore, the OECD agricultural policy-index system was used. To the extent that transversal criteria are applied, which support the transfer of aid between territories, the results suggest that changing the payment assignation model would involve a redistribution of aid towards areas that currently least benefit from... |
| Tipo: Journal article |
Palavras-chave: Agricultural policy; CAP post-2013; Direct payments; OECD; Redistribution. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000200005 |
| |
| Registros recuperados: 9.178 | |
|
|
|
